1. Enzymes are primarily:
2. The major function of an enzyme is to:
3. Most enzymes are chemically:
4. The protein portion of a conjugated enzyme is called:
5. Apoenzyme plus its required cofactor forms:
6. A cofactor that is tightly bound to an enzyme is called:
7. Which is an example of a coenzyme?
8. Coenzymes are generally:
11. The region of an enzyme where substrate binds is called:
12. Enzyme specificity is mainly due to the:
13. The lock-and-key model was proposed by:
14. The induced-fit model was proposed by:
15. In the induced-fit model, substrate binding:
17. Enzymes generally function best at a particular:
18. Extremely high temperature usually causes enzyme:
19. The inactive precursor of an enzyme is called:
20. Which is an example of a metal ion acting as a cofactor?
21. Enzymes usually show maximum activity at their:
22. Enzyme nomenclature and classification are standardized by:
23. The EC number of an enzyme represents its:
24. Oxidoreductases catalyse:
25. Transferases catalyse:
27. Lyases generally catalyse:
29. Ligases generally catalyse:
30. Enzyme catalytic activity is commonly expressed in:
31. One enzyme unit generally represents the amount of enzyme catalysing:
32. Enzyme specificity may be:
33. Proximity effect in enzyme catalysis refers to:
34. Orientation effects enhance catalysis by:
35. Acid-base catalysis involves:
36. Covalent catalysis involves formation of:
37. Metal ions can contribute to catalysis by:
38. The active form of an apoenzyme with its cofactor is:
39. The main objective of enzyme purification is to:
40. Enzyme purification usually begins with:
41. Cell disruption is necessary when the enzyme is:
42. Centrifugation separates components primarily according to:
43. Ammonium sulfate precipitation is an example of:
44. The solubility of proteins generally decreases at high:
45. Fractional precipitation means:
46. Dialysis is mainly used to remove:
47. Dialysis depends primarily on:
48. Chromatography separates molecules based on differences in:
49. Gel filtration chromatography separates proteins mainly according to:
50. Gel filtration is also known as:
51. In gel filtration, large molecules generally:
52. In gel filtration, small molecules:
53. lon-exchange chromatography separates proteins based primarily on:
54. A positively charged ion-exchange resin is called:
55. A negatively charged ion-exchange resin binds:
56. Anion-exchange chromatography binds:
57. Protein binding in ion-exchange chromatography depends strongly on:
58. Increasing ionic strength can often:
59. Affinity chromatography is based on:
60. Affinity chromatography generally provides:
